Jun 24, 2015 zheng cf, pan yt, cai wy, yuan xn, gao f 20 identification and determination of flavonoids in anoectochilus roxburghii wall. Optimization of culture conditions for the production of polysaccharides and kinsenoside from the rhizome cultures of anoectochilus roxburghii wall. Terrestrial whole plant consumed to treat tuberculosis ref. Molecules free fulltext optimization of ultrasound. Jan 19, 2018 the present study designed two sets of experiments by using the uniform design method and investigated the effects of medium components on the accumulation of bioactive compounds polysaccharide and kinsenoside in rhizomes, in order to select a suitable culture medium for the rhizome suspension culture of anoectochilus roxburghii wall. The species is distributed in india, china, nepal, bhutan, bangladesh, myanmar, thailand, laos, vietnam and japan. Pdf a novel flavonoid glucoside from anoectochilus roxburghii. Therefore, the aim of this study was to investigate the effects of drying methods on sensory qualities, aroma components. Uae of kinsenoside compound from anoectochilus roxburghii. Lindley 1839 transferred wallichs chrysobaphus roxburghii wallich, 1826 to the genus anoectochilus blume as anoectochilus roxburghii wall.
Ddiglucoside 3 extracted from anoectochilus roxburghii, has been studied through three main antioxidant pathways. The taxonomic distinction between anoectochilus and its close ally odontochilus has been problematic, and members of the latter and other closely related genera have frequently been lumped with those of the former. Several species of this genus have been used in chinese folk medicines, such as a. Chenseparation and purification of ergosterol and stigmasterol in anoectochilus roxburghii wall lindl by highspeed countercurrent chromatography j. Introduction lindley 1839 transferred wallichs chrysobaphus roxburghii wallich, 1826 to the genus anoectochilus blume as anoectochilus roxburghii wall. Kinsenoside and polysaccharide production by rhizome culture. The authors have done phot ographs and sketches of all the available orchid species of the region. Orchidaceae is a precious raw material for medicine. Comparative transcriptome analysis of genes involved in ga.
Cn102907327a tissue culture propagation method for. The genus anoectochilus orchidaceae is a perennial herb, which comprises more than 40 species that are widespread throughout tropical regions. Separation and purification of ergosterol and stigmasterol in anoectochilus roxburghii wall lindl by high. Anoectochilus roxburghii trends in biosciences journal. Anoectochilus, commonly known as marbled jewel orchids or filigree orchids, is a genus of about fifty species in the orchid family orchidaceae. Leaves purplish red with golden reticulations, velvety, ovate, the petioles short and somewhat expanded at the base, glabrous. A novel flavonoid glucoside from anoectochilus roxburghii wall. Pdf eight compounds were isolated from the ethyl acetate and.
Lindl tea, which is characterized by having a preparation process of. Uae of kinsenoside compound from anoectochilus wall. Culture of anoectochilus deep forest jewel orchids. There are no related plants for species anoectochilus roxburghii. Second name was found to be totally different plant and matched with the existing chrysobaphus roxburghii wall. Sensory qualities, aroma components, and bioactive. Feb 01, 2011 read separation and purification of ergosterol and stigmasterol in anoectochilus roxburghii wall lindl by high. Beneficial effects of endophytic fungi from the anoectochilus and. The invention discloses a tissue culture propagation method for anoectochilus roxburghii, and relates to a tissue culture method for plants. Optimum extraction conditions were determined as follows. Dna barcodes for the identification of anoectochilus.
Effects of the mycorrhizal fungus ceratobasidium sp. A small plant of the height between 7 to 15 cm of stem and 3 to 6 cm of inflorescence. Taxonomic assessment on the reported orchid species of. Orchidaceae, also known as the king medicine and golden herb, is a rare perennial herb that is mainly distributed in china, indian, nepal, japan and vietnam xu et al. In vitro endangered terrestrial jewel orchid anoectochilus. Radicalscavenging activity of isorhamnetin 1 and its diglycosides, named isorhamnetin3,5o.
This study used mae and rsm to extract phenolic compounds from anoectochilus roxburghii, and the optimum conditions defined by the model to give an optimum yield of 1. Extraction technology of total flavonoids of anoectochilus. Found in burma, thailand and malaysia in mountain forests at 3,600 to 5,000 ft 1200 to 1700 m. Pdf a note on the identity of anoectochilus roxburghii. Anoectochilus roxburghii orchidaceae, also known as jinxianlian simplified chinese. Lindl by response surface methodology rsm biyun yang 1,2, mengyuan zhang 1, haiyong weng 2, yong xu 2,3, and lihui zeng 1, 1 college of horticulture, fujian agriculture and forestry university, fuzhou 350002, china. Artificial propagation of anoectochilus roxburghii wall. Purification of polysaccharide from artificially cultivated. Bacillus subtilis bs87induced gene expression increased growth in plant is associated with the expression of growth marker genes. A novel flavonoid glucoside from anoectochilus roxburghii.
Uae of kinsenoside compound from anoectochilus roxburghii wall. Science china screening for differentially expressed. Antioxidant and hepatoprotective effects of anoectochilus. Artificial propagation is the only way for industrial application of this. Nghien c u nhan nhanh loai lan kim tuy n anoectochilus roxburghii. Anoectochilus roxburghii xe anoectochilus roxburghii wall. Researchers have found that kinsenoside had a di erent multiple pharmacologic activity compared with polysaccharide and. Lectotypification of the name chrysobaphus roxburghii. Collected specimens were made into standard mounted herbarium sheets following the procedure of jain and rao15. Beneficial effects of endophytic fungi from the anoectochilus. Anoectochilus is circumscribed here in line with pridgeon et al. A note on the identity of anoectochilus roxburghii orchidaceae. Terrestrial root root is used to relieve body ache ref. Sensory qualities, aroma components, and bioactive compounds.
Optimization of ultrasound assisted extraction uae of. First one based on priority of publication of icn is accepted and hence second name is rejected. The record derives from wcsp data supplied on 20120323 which reports it as an accepted name record 10233 with original publication details. Frontiers simultaneous extraction and identification of. The invention relates to an anoectochilus roxburghii wall. The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant anoectochilus roxburghii. The invention provides a tissue culture propagation method for anoectochilus roxburghii, through which the rapid propagation and growth of stem sections of anoectochilus roxburghii can be promoted quickly. Separation and purification of ergosterol and stigmasterol in. In vivo antioxidant experiments used dgalactose to. To provide rhizomes of as alternative plant materials, the present study used continuous immersion bioreactor systems.
They are terrestrial herbs with a creeping rhizome, an upright flowering stem and dark coloured leaves with contrasting veins. Mei yu jin, li qiu zhang, xuan chun piao, ri gao and mei lan lian. It was earlier considered as endemic but distributed. Lindl,extraction technology of total flavonoids was studied by orthogonal experiment. Theoretical study for exploring the diglycoside substituent. Sep 11, 2017 to establish a systematic method for the extraction, purification, characterization and antitumor activity study of polysaccharide from artificially cultivated anoectochilus roxburghii wall. To determine whether bs87 acts as an inducer of growth in a. Present status of orchid species diversity resources of. Ultrasound assisted extraction uae and ultrahigh performance liquid chromatography uplc method were used to extract and determine the content of kinsenoside, while response surface method rsm was used to optimize the extraction process. Promoting role of bacillus subtilis bs87 on the growth and. Article pdf available in journal of integrative plant biology 483. Common name roxburghs anoectochilus english botanical director in india 1800s in china jin xian lan in thailand wan mai na flower size less than 34 less than 1.
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